Hotspot

ycf1

SSC · 124386-129956 · 5571 bp

Back to Thalictrum

Pi 0.0135

MarkerSeek score 57.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5571
variable_sites: 447
pi: 0.0135
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTGCTATTGTTAGACATTCA	TTTCAAACAGCTGCGCATTC	33.3	45.0	52.3	58.2	206 bp	0.913043	44.5
ycf1_p2	TTCTGCTATTGTTAGACATTCA	TTTCAAACAGCTGCGCATTC	31.8	45.0	53.2	58.2	207 bp	0.913043	44.5
ycf1_p3	TGCTATTGTTAGACATTCATTTTCC	TTTCAAACAGCTGCGCATTC	32.0	45.0	56.2	58.2	204 bp	0.913043	44.5
ycf1_p4	CTGCTATTGTTAGACATTCATTTTCC	TTTCAAACAGCTGCGCATTC	34.6	45.0	57.2	58.2	205 bp	0.913043	44.5
ycf1_p5	TTTCTGCTATTGTTAGACATTCA	TTTCAAACAGCTGCGCATTC	30.4	45.0	54.0	58.2	208 bp	0.913043	44.5