Hotspot

ndhG-ndhI

SSC · 119195-119603 · 409 bp

Back to Thalictrum

Pi 0.0273

MarkerSeek score 73.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 409
variable_sites: 60
pi: 0.0273
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TACCACTCCTAGACCTCCCA	CAAATCACAAAAGCCACGGG	55.0	50.0	58.3	58.2	508-582 bp	0.869565	82.6
ndhG-ndhI_p2	CCACTCCTAGACCTCCCAAT	CAAATCACAAAAGCCACGGG	55.0	50.0	57.8	58.2	506-554 bp	0.826087	79.0
ndhG-ndhI_p3	CCACTCCTAGACCTCCCAAT	AAATCACAAAAGCCACGGGA	55.0	45.0	57.8	58.3	505-553 bp	0.826087	78.3
ndhG-ndhI_p4	CCACTCCTAGACCTCCCAAT	AATCACAAAAGCCACGGGAA	55.0	45.0	57.8	58.3	504-552 bp	0.826087	78.3
ndhG-ndhI_p5	CCACTCCTAGACCTCCCAAT	ATCACAAAAGCCACGGGAAA	55.0	45.0	57.8	58.3	503-551 bp	0.826087	78.3