Hotspot

ccsA-ndhD

SSC · 115734-115969 · 236 bp

Back to Thalictrum

Pi 0.0354

MarkerSeek score 73.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 236
variable_sites: 38
pi: 0.0354
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAATTGTGGCTTCTATGGG	TCCGGATTTCGTTCTCTCAC	50.0	50.0	57.1	57.4	387-430 bp	1.0	79.8
ccsA-ndhD_p2	GCAATTGTGGCTTCTATGGG	CCGGATTTCGTTCTCTCACT	50.0	50.0	57.1	57.3	386-429 bp	1.0	79.7
ccsA-ndhD_p3	GGGCTGCATAGTTATGGTTCA	TCCGGATTTCGTTCTCTCAC	47.6	50.0	58.1	57.4	318-361 bp	1.0	78.3
ccsA-ndhD_p4	GGGCTGCATAGTTATGGTTCA	CCGGATTTCGTTCTCTCACT	47.6	50.0	58.1	57.3	317-360 bp	1.0	78.2
ccsA-ndhD_p5	AGGGCTGCATAGTTATGGTTC	TCCGGATTTCGTTCTCTCAC	47.6	50.0	57.8	57.4	319-362 bp	1.0	77.1