Hotspot

ndhF-trnL-UAG

SSC · 113823-114563 · 741 bp

Back to Thalictrum

Pi 0.0331

MarkerSeek score 81.30

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 741
variable_sites: 129
pi: 0.0331
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	CAAGGAGAAGACCCATCCCT	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.4	58.0	855-1322 bp	0.956522	86.9
ndhF-trnL-UAG_p2	CAAGGAGAAGACCCATCCCT	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.4	58.0	856-1323 bp	0.956522	86.9
ndhF-trnL-UAG_p3	CAAGGAGAAGACCCATCCCT	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.4	57.9	865-1332 bp	0.956522	86.0
ndhF-trnL-UAG_p4	CAAGGAGAAGACCCATCCCT	TTATAAGATGCCGCCACTCG	55.0	50.0	58.4	57.9	914-1381 bp	0.956522	85.8
ndhF-trnL-UAG_p5	CAAGGAGAAGACCCATCCCT	CTAAGAGCAGCGTGTCTACC	55.0	55.0	58.4	57.8	863-1330 bp	0.956522	85.4