Hotspot

psbE-petL

LSC · 65463-66542 · 1080 bp

Back to Thalictrum

Pi 0.00817

MarkerSeek score 69.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1080
variable_sites: 60
pi: 0.00817
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CCAACCTGCAATGAATAGCG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.5	57.1	979-1300 bp	1.0	76.7
psbE-petL_p2	CAAAAGAACGTTCTCCCGTG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.3	57.1	905-1226 bp	1.0	75.4
psbE-petL_p3	CAGCAAAAGAACGTTCTCCC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.3	57.1	908-1229 bp	1.0	75.2
psbE-petL_p4	CCAACCTGCAATGAATAGCG	GTCGTATCTTGCTCAGACCAA	50.0	47.6	57.5	57.8	979-1300 bp	1.0	74.4
psbE-petL_p5	TGACGAATAACCAACCTGCA	GTCGTATCTTGCTCAGACCA	45.0	50.0	57.4	57.1	1263-1310 bp	0.913043	69.5