Hotspot

ndhC-trnV-UAC

LSC · 51504-52447 · 944 bp

Back to Thalictrum

Pi 0.023

MarkerSeek score 80.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 110
pi: 0.023
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTGGTCCCTCGTTAATTGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	1015-1124 bp	1.0	90.1
ndhC-trnV-UAC_p2	GGGGCCAAAACTCCAGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	998-1107 bp	1.0	87.6
ndhC-trnV-UAC_p3	CTGGTCCCTCGTTAATTGGG	GAAGGTCTACGGTTCGAGTC	55.0	55.0	58.0	57.5	1014-1123 bp	1.0	87.4
ndhC-trnV-UAC_p4	CCTCGTTAATTGGGGCCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1009-1118 bp	1.0	86.8
ndhC-trnV-UAC_p5	CTGGTCCCTCGTTAATTGGG	GTTCGAGTCCGTATAGCCCT	55.0	55.0	58.0	58.7	1003-1112 bp	1.0	86.2