Hotspot

rps4-trnT-UGU

LSC · 47029-47412 · 384 bp

Back to Thalictrum

Pi 0.0153

MarkerSeek score 65.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 384
variable_sites: 37
pi: 0.0153
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GGGTGTCGGCCTTTTACTAG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.0	57.9	518-560 bp	1.0	88.6
rps4-trnT-UGU_p2	GGGTGTCGGCCTTTTACTAG	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.0	57.8	486-528 bp	1.0	88.1
rps4-trnT-UGU_p3	GGGTGTCGGCCTTTTACTAG	TTTATGTGAGCCCGCTTAGC	55.0	50.0	58.0	58.3	537-579 bp	1.0	86.9
rps4-trnT-UGU_p4	TTCTGAGATCACTTCCGGGT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	534-576 bp	0.913043	84.2
rps4-trnT-UGU_p5	TTCTGAGATCACTTCCGGGT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	502-544 bp	0.913043	83.7