Hotspot

trnS-UGA-psbZ

LSC · 36643-36996 · 354 bp

Back to Thalictrum

Pi 0.0223

MarkerSeek score 71.70

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 354
variable_sites: 41
pi: 0.0223
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCGTCAGAAGAAGCAA	55.0	50.0	58.0	58.4	468-503 bp	1.0	85.0
trnS-UGA-psbZ_p2	GAGCTATCAACCACTCAGCC	CCAACCGTCAGAAGAAGCAA	55.0	50.0	58.1	58.4	475-510 bp	1.0	84.6
trnS-UGA-psbZ_p3	CAACCACTCAGCCATCTCTC	TGACCAACCGTCAGAAGAAG	55.0	50.0	58.0	57.5	471-506 bp	1.0	84.0
trnS-UGA-psbZ_p4	CGGTTTTCAAGACCAGAGCT	CCAACCGTCAGAAGAAGCAA	50.0	50.0	58.1	58.4	490-525 bp	1.0	83.8
trnS-UGA-psbZ_p5	GAGCTATCAACCACTCAGCC	TGACCAACCGTCAGAAGAAG	55.0	50.0	58.1	57.5	478-513 bp	1.0	83.6