Hotspot

trnT-GGU-psbD

LSC · 32688-33915 · 1228 bp

Back to Thalictrum

Pi 0.0092

MarkerSeek score 68.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1228
variable_sites: 66
pi: 0.0092
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	TGTCTTCACGTTGGCTCATT	ACAAAAACGAAACGGTCCCT	45.0	45.0	57.7	58.2	1400-1506 bp	1.0	83.6
trnT-GGU-psbD_p2	TGTCTTCACGTTGGCTCATT	GACCGGACCAACCTACAAAA	45.0	50.0	57.7	57.7	1414-1520 bp	1.0	83.5
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1330-1436 bp	1.0	83.5
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	GACCGGACCAACCTACAAAA	50.0	50.0	58.3	57.7	1344-1450 bp	1.0	83.3
trnT-GGU-psbD_p5	CCCCTTTAACTCAGCGGTAG	ACAAAAACGAAACGGTCCCT	55.0	45.0	57.7	58.2	1366-1472 bp	1.0	83.2