Hotspot

psbM-trnD-GUC

LSC · 30033-31173 · 1141 bp

Back to Thalictrum

Pi 0.00952

MarkerSeek score 70.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1141
variable_sites: 67
pi: 0.00952
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.3	58.5	1227-1257 bp	1.0	82.5
psbM-trnD-GUC_p2	TGAACAGCGCAGTAGCAATA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	1226-1256 bp	1.0	81.5
psbM-trnD-GUC_p3	AATGAACAGCGCAGTAGCAA	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.5	58.5	1228-1258 bp	1.0	80.7
psbM-trnD-GUC_p4	AGAATGAACAGCGCAGTAGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.6	58.5	1230-1260 bp	1.0	79.4
psbM-trnD-GUC_p5	ACTAGAATGAACAGCGCAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.2	58.5	1233-1263 bp	1.0	78.5