Hotspot

petN-psbM

LSC · 28723-29927 · 1205 bp

Back to Thalictrum

Pi 0.0139

MarkerSeek score 75.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1205
variable_sites: 96
pi: 0.0139
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	ATTGCTACTGCGCTGTTCAT	45.0	45.0	58.0	58.3	1328-1370 bp	1.0	87.7
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	TATTGCTACTGCGCTGTTCA	45.0	45.0	58.0	57.6	1329-1371 bp	1.0	86.7
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	TTGCTACTGCGCTGTTCATT	45.0	45.0	58.0	58.5	1327-1369 bp	1.0	85.9
petN-psbM_p4	AGTAAGTCTTGCTTGGGCTG	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.8	58.3	1334-1376 bp	0.956522	84.3
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TATTGCTACTGCGCTGTTCA	50.0	45.0	57.8	57.6	1335-1377 bp	0.956522	83.4