Hotspot

atpF-atpH

LSC · 12649-13103 · 455 bp

Back to Thalictrum

Pi 0.0246

MarkerSeek score 67.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 48
pi: 0.0246
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	TCGTGGCATTAGCGCTTTTA	50.0	45.0	58.0	58.3	488-553 bp	1.0	88.8
atpF-atpH_p2	CAGTGACCCAAGGAAACGAA	CGTGGCATTAGCGCTTTTAT	50.0	45.0	58.0	57.2	487-552 bp	1.0	85.0
atpF-atpH_p3	AGTGACCCAAGGAAACGAAA	TCGTGGCATTAGCGCTTTTA	45.0	45.0	57.3	58.3	487-552 bp	1.0	83.4
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	CGTGGCATTAGCGCTTTTATT	50.0	42.9	58.0	57.9	487-552 bp	1.0	82.5
atpF-atpH_p5	TGACCCAAGGAAACGAAAGA	TCGTGGCATTAGCGCTTTTA	45.0	45.0	57.0	58.3	485-550 bp	1.0	81.1