Hotspot

rps16-trnQ-UUG

LSC · 5850-6879 · 1030 bp

Back to Thalictrum

Pi 0.0147

MarkerSeek score 71.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1030
variable_sites: 98
pi: 0.0147
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCACAACCGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1068-1160 bp	1.0	89.5
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1047-1139 bp	1.0	89.4
rps16-trnQ-UUG_p3	CCACAACCGATCATGTCCTT	CTCGGAGGTTCGAATCCTTC	50.0	55.0	57.9	57.8	1072-1164 bp	1.0	88.3
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1051-1143 bp	1.0	88.3
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1058-1150 bp	0.956522	86.8