Hotspot

trnK-UUU-rps16

LSC · 4163-4733 · 571 bp

Back to Thalictrum

Pi 0.0191
MarkerSeek score 64.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
571
variable_sites
54
pi
0.0191
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CACATTCGTCTCCACTCAGG TCGATCCCGAAGAGAAGGAA 55.0 50.0 58.0 57.9 1245-1437 bp 1.0 87.4
trnK-UUU-rps16_p2 TCAGGATCAGTCGTGGTCTT TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.1 57.9 873-1066 bp 1.0 87.2
trnK-UUU-rps16_p3 CACATTCGTCTCCACTCAGG ATTCTTTCTCGAGCCGTACG 55.0 50.0 58.0 57.8 1358-1549 bp 1.0 86.8
trnK-UUU-rps16_p4 AGGATAGGGGATTTCTCGGG TCGATCCCGAAGAGAAGGAA 55.0 50.0 58.0 57.9 1510-1679 bp 0.913043 81.2
trnK-UUU-rps16_p5 CACATTCGTCTCCACTCAGG TGAAATGCCAAGGACTCAGG 55.0 50.0 58.0 57.8 1559-1737 bp 0.782609 74.4