Hotspot

ycf90

Genome · 58449-59870 · 1422 bp

Back to Thalassiosira

Pi 0.143

MarkerSeek score 79.30

Primer available yes

Label ycf90

Feature Metadata

label_name: ycf90
feature_type: gene
region: Genome
length_bp: 1422
variable_sites: 203
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf90_p1	TACACAAATGGGTGAACCGG	TTAGTAGGTGGTTTCGCAGC	50.0	50.0	58.1	57.9	2107-2108 bp	1.0	86.7
ycf90_p2	TACACAAATGGGTGAACCGG	GGTGGTTTCGCAGCAATAAC	50.0	50.0	58.1	58.0	2101 bp	0.5	59.0
ycf90_p3	ATGGCACCTACACAAATGGG	GGTGGTTTCGCAGCAATAAC	50.0	50.0	58.1	58.0	2109 bp	0.5	58.6
ycf90_p4	TACACAAATGGGTGAACCGG	TCGTTGGTTAGCAGTCCATG	50.0	50.0	58.1	57.9	2407 bp	0.5	58.4
ycf90_p5	TACACAAATGGGTGAACCGG	AGGTGGTTTCGCAGCAATAA	50.0	45.0	58.1	58.1	2102 bp	0.5	58.4