Hotspot

rps4

Genome · 84557-85174 · 618 bp

Back to Thalassiosira

Pi 0.11

MarkerSeek score 79.00

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: Genome
length_bp: 618
variable_sites: 68
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	CTACCATCTCGTCGGGTAGT	GTTAAGGCAGTGGGTTGTGA	55.0	50.0	58.0	58.0	903 bp	0.5	59.5
rps4_p2	CTACCATCTCGTCGGGTAGT	GTGGGTTGTGATTCCACCAT	55.0	50.0	58.0	58.1	894 bp	0.5	59.1
rps4_p3	CGTCGGGTAGTGTTTTCCAT	GTTAAGGCAGTGGGTTGTGA	50.0	50.0	57.9	58.0	894 bp	0.5	59.1
rps4_p4	CGTCGGGTAGTGTTTTCCAT	GTGGGTTGTGATTCCACCAT	50.0	50.0	57.9	58.1	885 bp	0.5	58.7
rps4_p5	CTACCATCTCGTCGGGTAGT	CACTCGCCCTACTTCAAACA	55.0	50.0	58.0	57.8	855 bp	0.5	58.4