Hotspot

psaE

Genome · 19831-20028 · 198 bp

Back to Thalassiosira

Pi 0.255

MarkerSeek score 75.40

Primer available yes

Label psaE

Feature Metadata

label_name: psaE
feature_type: gene
region: Genome
length_bp: 198
variable_sites: 49
pi: 0.255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaE_p1	CAAGCAGAAGGTCACAGGTT	ACAAGCTTGTCCTGGGTTTT	50.0	45.0	58.0	57.8	1109-1158 bp	1.0	90.1
psaE_p2	GCAGAAGGTCACAGGTTCAA	ACAAGCTTGTCCTGGGTTTT	50.0	45.0	58.0	57.8	1106-1155 bp	1.0	90.1
psaE_p3	TCGTCTAACGGATCAGGACA	ACAAGCTTGTCCTGGGTTTT	50.0	45.0	57.9	57.8	1051-1100 bp	1.0	89.6
psaE_p4	AACTCAGCGGTAGAGTGTCT	ACAAGCTTGTCCTGGGTTTT	50.0	45.0	58.1	57.8	1135-1184 bp	1.0	89.5
psaE_p5	GAGTGTCTGCCTTACAAGCA	ACAAGCTTGTCCTGGGTTTT	50.0	45.0	57.8	57.8	1123-1172 bp	1.0	88.9