Hotspot

dnaB

Genome · 23848-25209 · 1362 bp

Back to Thalassiosira

Pi 0.108

MarkerSeek score 80.30

Primer available yes

Label dnaB

Feature Metadata

label_name: dnaB
feature_type: gene
region: Genome
length_bp: 1362
variable_sites: 147
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaB_p1	TCAGTTGGTAGAGCAGTGGA	GGACTTGAACCCTCGACATC	50.0	55.0	58.0	58.0	1571-1577 bp	1.0	87.6
dnaB_p2	TCAGTTGGTAGAGCAGTGGA	GAAGGACTTGAACCCTCGAC	50.0	55.0	58.0	57.9	1574-1580 bp	1.0	87.0
dnaB_p3	TCAGTTGGTAGAGCAGTGGA	CTGAAGGACTTGAACCCTCG	50.0	55.0	58.0	57.9	1576-1582 bp	1.0	87.0
dnaB_p4	ACGAAACCCAATCAACGACA	GGACTTGAACCCTCGACATC	45.0	55.0	58.0	58.0	2150 bp	0.5	59.6
dnaB_p5	TCAGTTGGTAGAGCAGTGGA	AGGTGCAGGTGTAGCTTTTC	50.0	50.0	58.0	58.1	1843 bp	0.5	59.1