Hotspot

trnI-GAU-rns

Genome · 126437-126495 · 59 bp

Back to Thalassiosira

Pi 0.103

MarkerSeek score 79.70

Primer available yes

Label trnI-GAU-rns

Feature Metadata

label_name: trnI-GAU-rns
feature_type: igs
region: Genome
length_bp: 59
variable_sites: 6
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-rns_p1	TTATCAGGGGTGCGCTCTAA	GACTGGGGTGAAGTCGTAAC	50.0	55.0	58.5	57.9	158-159 bp	1.0	78.9
trnI-GAU-rns_p2	TTATCAGGGGTGCGCTCTAA	GGAGGGTGCCTAAGGTAGAA	50.0	55.0	58.5	58.1	183-184 bp	1.0	78.0
trnI-GAU-rns_p3	TTATCAGGGGTGCGCTCTAA	GGGGTGAAGTCGTAACAAGG	50.0	55.0	58.5	58.2	154-155 bp	1.0	78.0
trnI-GAU-rns_p4	TGGGCCATTCTGGATTTGAA	GACTGGGGTGAAGTCGTAAC	45.0	55.0	57.4	57.9	193-194 bp	1.0	77.3
trnI-GAU-rns_p5	TTATCAGGGGTGCGCTCTAA	TCTAACCATTCGGAGGAGGG	50.0	55.0	58.5	58.2	197-198 bp	1.0	77.1