Hotspot

groEL-dnaK

Genome · 88417-88900 · 484 bp

Back to Thalassiosira

Pi 0.413
MarkerSeek score 79.60
Primer available yes
Label groEL-dnaK

Feature Metadata

label_name
groEL-dnaK
feature_type
igs
region
Genome
length_bp
484
variable_sites
177
pi
0.413
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
groEL-dnaK_p1 CTGTCATTCGTGTAGGTGCA GCAGGTATTTTAGCGGGTGA 50.0 50.0 57.9 58.0 1629 bp 0.5 59.1
groEL-dnaK_p2 TGCTGTCATTCGTGTAGGTG GCAGGTATTTTAGCGGGTGA 50.0 50.0 57.9 58.0 1631 bp 0.5 59.1
groEL-dnaK_p3 TGTCATTCGTGTAGGTGCAG GCAGGTATTTTAGCGGGTGA 50.0 50.0 57.9 58.0 1628 bp 0.5 59.1
groEL-dnaK_p4 GTCATTCGTGTAGGTGCAGT GCAGGTATTTTAGCGGGTGA 50.0 50.0 57.9 58.0 1627 bp 0.5 59.1
groEL-dnaK_p5 TGTCATTCGTGTAGGTGCAG AGCAGGTATTTTAGCGGGTG 50.0 50.0 57.9 58.0 1629 bp 0.5 59.1