Hotspot

trnQ-UUG-trnR-ACG

Genome · 86517-86584 · 68 bp

Back to Thalassiosira

Pi 0.132
MarkerSeek score 79.00
Primer available yes
Label trnQ-UUG-trnR-ACG

Feature Metadata

label_name
trnQ-UUG-trnR-ACG
feature_type
igs
region
Genome
length_bp
68
variable_sites
9
pi
0.132
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-trnR-ACG_p1 AAAGTCCACAGCCTTACCAC GGGGTTCGAATCCCTTCTTG 50.0 55.0 57.7 58.2 128 bp 1.0 81.5
trnQ-UUG-trnR-ACG_p2 CAAAGTCCACAGCCTTACCA GGGGTTCGAATCCCTTCTTG 50.0 55.0 57.7 58.2 129 bp 1.0 81.4
trnQ-UUG-trnR-ACG_p3 GAGTCAAAGTCCACAGCCTT GGGGTTCGAATCCCTTCTTG 50.0 55.0 57.7 58.2 133 bp 1.0 81.3
trnQ-UUG-trnR-ACG_p4 TTAAAAACTGGGGTGGCAGG GGGGTTCGAATCCCTTCTTG 50.0 55.0 58.3 58.2 173 bp 1.0 79.9
trnQ-UUG-trnR-ACG_p5 GTGGCAGGATTCGAACCTAC GGGGTTCGAATCCCTTCTTG 55.0 55.0 58.3 58.2 161 bp 1.0 79.7