Hotspot

thiS-psb28

Genome · 85617-86013 · 397 bp

Back to Thalassiosira

Pi 0.259

MarkerSeek score 75.30

Primer available yes

Label thiS-psb28

Feature Metadata

label_name: thiS-psb28
feature_type: igs
region: Genome
length_bp: 397
variable_sites: 102
pi: 0.259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiS-psb28_p1	TCACAACCCACTGCCTTAAC	CTTTGACTCCGCCATTCGTA	50.0	50.0	58.0	58.0	1206-1922 bp	1.0	87.9
thiS-psb28_p2	TCACAACCCACTGCCTTAAC	TTTGACTCCGCCATTCGTAG	50.0	50.0	58.0	58.0	1205-1921 bp	1.0	87.9
thiS-psb28_p3	TCACAACCCACTGCCTTAAC	AGGGATGGAAGTACTGGGAC	50.0	55.0	58.0	58.1	1007 bp	0.5	58.9
thiS-psb28_p4	ACCAGTTACACCGTTTCGTC	CTTTGACTCCGCCATTCGTA	50.0	50.0	58.1	58.0	1566 bp	0.5	58.8
thiS-psb28_p5	ACCAGTTACACCGTTTCGTC	TTTGACTCCGCCATTCGTAG	50.0	50.0	58.1	58.0	1565 bp	0.5	58.8