Hotspot

ycf45-ffs

Genome · 22856-23576 · 721 bp

Back to Thalassiosira

Pi 0.424

MarkerSeek score 84.40

Primer available yes

Label ycf45-ffs

Feature Metadata

label_name: ycf45-ffs
feature_type: igs
region: Genome
length_bp: 721
variable_sites: 278
pi: 0.424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf45-ffs_p1	TACTCAGCCTTTCAGCTTGC	TCCACTGCTCTACCAACTGA	50.0	50.0	58.2	58.0	1477 bp	0.5	58.4
ycf45-ffs_p2	AGAAGCTAAACGGAGAGGGA	TCCACTGCTCTACCAACTGA	50.0	50.0	57.8	58.0	1526 bp	0.5	58.1
ycf45-ffs_p3	TACTCAGCCTTTCAGCTTGC	CATCGTAATGGTCCGCTAGG	50.0	55.0	58.2	57.9	1601 bp	0.5	58.0
ycf45-ffs_p4	AGAAGCTAAACGGAGAGGGA	CATCGTAATGGTCCGCTAGG	50.0	55.0	57.8	57.9	1650 bp	0.5	57.6
ycf45-ffs_p5	GAAGCTAAACGGAGAGGGAC	TCCACTGCTCTACCAACTGA	55.0	50.0	57.7	58.0	1525 bp	0.5	57.5