Hotspot

ycf1

SSC · 62849-68059 · 5211 bp

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Pi 0.0668

MarkerSeek score 78.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5211
variable_sites: 712
pi: 0.0668
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAAACTCCTCCCAGACCTCT	TCATCCTCAACGCCCAAAAA	55.0	45.0	58.1	58.0	745 bp	0.333333	53.0
ycf1_p2	GAAACTCCTCCCAGACCTCT	TTCATCCTCAACGCCCAAAA	55.0	45.0	58.1	58.0	746 bp	0.333333	53.0
ycf1_p3	GAAACTCCTCCCAGACCTCT	TTTCATCCTCAACGCCCAAA	55.0	45.0	58.1	58.0	747 bp	0.333333	53.0
ycf1_p4	GGGAATGCGCATTTGTTTGA	TCATCCTCAACGCCCAAAAA	45.0	45.0	57.9	58.0	535 bp	0.333333	52.9
ycf1_p5	GGGAATGCGCATTTGTTTGA	TTCATCCTCAACGCCCAAAA	45.0	45.0	57.9	58.0	536 bp	0.333333	52.9