Hotspot

ndhA

SSC · 58733-60906 · 2174 bp

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Pi 0.0136

MarkerSeek score 59.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2174
variable_sites: 60
pi: 0.0136
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACTGAGTTAGCATAGGGAACA	GCCGGGTTTTATCAACTTGC	42.9	50.0	56.1	58.0	2286-2392 bp	1.0	62.6
ndhA_p2	GAACTGAGTTAGCATAGGGAACA	GCCGGGTTTTATCAACTTGC	43.5	50.0	57.9	58.0	2288-2394 bp	1.0	61.3
ndhA_p3	ACTGAGTTAGCATAGGGAACA	TGCAAATTCTTCCGCAGCTA	42.9	45.0	56.1	58.2	2269-2375 bp	1.0	61.2
ndhA_p4	ACTGAGTTAGCATAGGGAACA	CGCCGGGTTTTATCAACTTG	42.9	50.0	56.1	57.7	2287-2393 bp	1.0	60.6
ndhA_p5	AACTGAGTTAGCATAGGGAACA	GCCGGGTTTTATCAACTTGC	40.9	50.0	56.8	58.0	2287-2393 bp	1.0	60.5