Hotspot

atpF-atpH

LSC · 105522-105875 · 354 bp

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Pi 0.333

MarkerSeek score 51.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 354
variable_sites: 1
pi: 0.333
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	CCTGAGGCAGAGGGAAAAAT	45.0	50.0	57.9	57.5	511-515 bp	0.5	59.5
atpF-atpH_p2	GTGGCCCAAAGAAACGAAAG	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	57.9	57.5	512-516 bp	0.5	58.9
atpF-atpH_p3	AGTGGCCCAAAGAAACGAAA	CCTGAGGCAGAGGGAAAAAT	45.0	50.0	58.2	57.5	513-517 bp	0.5	58.1
atpF-atpH_p4	TGGCCCAAAGAAACGAAAGA	GCAGAGGGAAAAATACGAGGT	45.0	47.6	57.9	58.0	505-509 bp	0.5	55.6
atpF-atpH_p5	TTAAATCCGAAACTCCCGGC	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	58.3	57.5	541-542 bp	0.333333	51.1