Hotspot

rpl32-trnL-UAG

SSC · 52887-53324 · 438 bp

Back to Teucrium

Pi 0.0384

MarkerSeek score 67.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 438
variable_sites: 31
pi: 0.0384
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTGCGACAAACAAATAAACT	TCCTAAGAGCAGCGTGTCTA	33.3	50.0	54.9	57.9	479-515 bp	1.0	55.3
rpl32-trnL-UAG_p2	TGTGCGACAAACAAATAAACT	AGCAGCGTGTCTACCAATTT	33.3	45.0	54.9	57.8	472-508 bp	1.0	54.9
rpl32-trnL-UAG_p3	TGTGCGACAAACAAATAAACT	CCTAAGAGCAGCGTGTCTAC	33.3	55.0	54.9	57.8	478-514 bp	1.0	54.7
rpl32-trnL-UAG_p4	TGTGCGACAAACAAATAAACT	CTAAGAGCAGCGTGTCTACC	33.3	55.0	54.9	57.8	477-513 bp	1.0	54.7
rpl32-trnL-UAG_p5	TGTGCGACAAACAAATAAACT	GAGCAGCGTGTCTACCAATT	33.3	50.0	54.9	58.3	473-509 bp	1.0	54.2