Hotspot

trnP-UGG-psaJ

LSC · 8589-8871 · 283 bp

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Pi 0.0669

MarkerSeek score 78.00

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 283
variable_sites: 53
pi: 0.0669
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	AATAGACCTGCTAAAGCCGC	50.0	50.0	58.1	58.0	415-466 bp	1.0	89.6
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	AATAGACCTGCTAAAGCCGC	50.0	50.0	58.1	58.0	414-465 bp	1.0	89.6
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	AATAGACCTGCTAAAGCCGC	50.0	50.0	58.1	58.0	426-477 bp	1.0	89.5
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	AATAGACCTGCTAAAGCCGC	50.0	50.0	58.1	58.0	425-476 bp	1.0	89.5
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	AATAGACCTGCTAAAGCCGC	50.0	50.0	58.1	58.0	428-479 bp	1.0	89.5