Hotspot

petA-psbJ

LSC · 5221-6065 · 845 bp

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Pi 0.0124

MarkerSeek score 69.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 845
variable_sites: 21
pi: 0.0124
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCTTGT	50.0	47.6	58.2	57.4	901-929 bp	1.0	71.9
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCTTGT	50.0	47.6	58.2	57.4	903-931 bp	1.0	71.9
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	CCGGATTAGGTTCATCCTTGT	45.0	47.6	57.7	57.4	900-928 bp	1.0	71.1
petA-psbJ_p4	CGTGTCCAAGGTCTTTTGGT	CCGGATTAGGTTCATCCTTGT	50.0	47.6	58.3	57.4	979-1007 bp	1.0	70.6
petA-psbJ_p5	GAGAAGGTTCAATTGGCCGA	GCCGATACTACTGGAAGGAT	50.0	50.0	58.2	56.2	998-1026 bp	1.0	69.9