Hotspot

ycf4-cemA

LSC · 2695-3363 · 669 bp

Back to Teucrium

Pi 0.0242

MarkerSeek score 65.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 669
variable_sites: 32
pi: 0.0242
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GTTTATGCTCGTCGTGTCCT	TTCGGATTGCCCAGTATTCG	50.0	50.0	58.0	58.1	913-954 bp	1.0	87.2
ycf4-cemA_p2	AATCGTCGCATATTCCTCCG	TTCGGATTGCCCAGTATTCG	50.0	50.0	57.9	58.1	982-1023 bp	1.0	86.3
ycf4-cemA_p3	CGTCGCATATTCCTCCGATT	TTCGGATTGCCCAGTATTCG	50.0	50.0	57.9	58.1	979-1020 bp	1.0	86.3
ycf4-cemA_p4	AACTTATAGCGGGGTCTCGA	TTCGGATTGCCCAGTATTCG	50.0	50.0	57.9	58.1	1287-1328 bp	1.0	85.7
ycf4-cemA_p5	ACTTATAGCGGGGTCTCGAA	TTCGGATTGCCCAGTATTCG	50.0	50.0	57.9	58.1	1286-1327 bp	1.0	85.7