Hotspot

ndhF

Genome · 112354-114591 · 2238 bp

Back to Tetrataenium

Pi 0.00496

MarkerSeek score 47.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2238
variable_sites: 11
pi: 0.00496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTGAGGCTCTAGATACGGGA	TTGAAAGGGGGTAGGATCCG	50.0	55.0	57.0	58.8	2846-2922 bp	1.0	68.3
ndhF_p2	TTGAGGCTCTAGATACGGGA	GGGGTAGGATCCGGTAACTC	50.0	60.0	57.0	58.7	2915 bp	0.5	46.3
ndhF_p3	TGAGGCTCTAGATACGGGAT	GGGGTAGGATCCGGTAACTC	50.0	60.0	56.7	58.7	2914 bp	0.5	44.5
ndhF_p4	AAATTGGAAGGGCCTCTCTT	GGGGTAGGATCCGGTAACTC	45.0	60.0	56.7	58.7	2584 bp	0.5	44.2
ndhF_p5	AATTGGAAGGGCCTCTCTTT	GGGGTAGGATCCGGTAACTC	45.0	60.0	56.7	58.7	2583 bp	0.5	44.2