Hotspot

rrn16-trnH-GUG

Genome · 140269-141473 · 1205 bp

Back to Tetrataenium

Pi 0.0463

MarkerSeek score 67.20

Primer available yes

Label rrn16-trnH-GUG

Feature Metadata

label_name: rrn16-trnH-GUG
feature_type: igs
region: Genome
length_bp: 1205
variable_sites: 33
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.59
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnH-GUG_p1	CCGACTTGCATGTGTTAAGC	GATTGTGAATCCACCATGCG	50.0	50.0	57.8	57.5	1315 bp	0.5	54.2
rrn16-trnH-GUG_p2	GCAGGTTCTTACGCGTTACT	GATTGTGAATCCACCATGCG	50.0	50.0	58.3	57.5	1368 bp	0.5	53.8
rrn16-trnH-GUG_p3	TTCTTACGCGTTACTCACCC	GATTGTGAATCCACCATGCG	50.0	50.0	57.6	57.5	1363 bp	0.5	53.3
rrn16-trnH-GUG_p4	CCGACTTGCATGTGTTAAGC	TCAAGGCAGTGGATTGTGAA	50.0	45.0	57.8	57.3	1326 bp	0.5	52.8
rrn16-trnH-GUG_p5	GCAGGTTCTTACGCGTTACT	TCAAGGCAGTGGATTGTGAA	50.0	45.0	58.3	57.3	1379 bp	0.5	52.4