Hotspot

trnH-GUG-rrn16

Genome · 100628-101832 · 1205 bp

Back to Tetrataenium

Pi 0.0705
MarkerSeek score 82.90
Primer available yes
Label trnH-GUG-rrn16

Feature Metadata

label_name
trnH-GUG-rrn16
feature_type
igs
region
Genome
length_bp
1205
variable_sites
85
pi
0.0705
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-rrn16_p1 CGGATGTAGCCAAGTGGATC CCGACTTGCATGTGTTAAGC 55.0 50.0 58.1 57.8 1344-1684 bp 1.0 81.5
trnH-GUG-rrn16_p2 TGGATCAAGGCAGTGGATTG CCGACTTGCATGTGTTAAGC 50.0 50.0 57.9 57.8 1330-1670 bp 1.0 81.5
trnH-GUG-rrn16_p3 GGATCAAGGCAGTGGATTGT CCGACTTGCATGTGTTAAGC 50.0 50.0 57.9 57.8 1329-1669 bp 1.0 81.5
trnH-GUG-rrn16_p4 GTGGATCAAGGCAGTGGATT CCGACTTGCATGTGTTAAGC 50.0 50.0 57.9 57.8 1331-1671 bp 1.0 81.5
trnH-GUG-rrn16_p5 CGGATGTAGCCAAGTGGATC GCAGGTTCTTACGCGTTACT 55.0 50.0 58.1 58.3 1397-1737 bp 1.0 81.0