Hotspot

petA-psbJ

Genome · 63348-64300 · 953 bp

Back to Tetrataenium

Pi 0.0336
MarkerSeek score 78.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
Genome
length_bp
953
variable_sites
32
pi
0.0336
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CTTACGTGTCCAAGGCCTTT GCTGGTATTCTTGTGATCGGT 50.0 47.6 58.1 58.1 1143-1165 bp 1.0 72.7
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC GCTGGTATTCTTGTGATCGGT 50.0 47.6 57.8 58.1 1130-1152 bp 1.0 72.4
petA-psbJ_p3 CTTACGTGTCCAAGGCCTTT AGCTGGTATTCTTGTGATCGG 50.0 47.6 58.1 57.8 1144-1166 bp 1.0 72.0
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC AGCTGGTATTCTTGTGATCGG 50.0 47.6 57.8 57.8 1131-1153 bp 1.0 71.7
petA-psbJ_p5 GATCCCTTACGTGTCCAAGG GCTGGTATTCTTGTGATCGGT 55.0 47.6 57.7 58.1 1148-1170 bp 1.0 71.1