Hotspot

trnE-UUC-trnT-GGU

Genome · 30704-31475 · 772 bp

Back to Tetrataenium

Pi 0.012
MarkerSeek score 68.00
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
Genome
length_bp
772
variable_sites
9
pi
0.012
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 874-876 bp 1.0 81.2
trnE-UUC-trnT-GGU_p2 GTGTAGTACCCTACCCCCAG GAACCGATGACTTACGCCTT 60.0 50.0 58.2 58.0 909-911 bp 1.0 80.7
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA GGCGTTACTCTACCGTTGAG 50.0 55.0 57.8 58.1 849-851 bp 1.0 80.5
trnE-UUC-trnT-GGU_p4 GTGTAGTACCCTACCCCCAG GGCGTTACTCTACCGTTGAG 60.0 55.0 58.2 58.1 884-886 bp 1.0 80.1
trnE-UUC-trnT-GGU_p5 CGTTGCCTCCTTGAAAGAGA CCGATGACTTACGCCTTACC 50.0 55.0 57.8 58.2 871-873 bp 1.0 80.0