Hotspot

rps2-rpoC2

Genome · 15585-15804 · 220 bp

Back to Tetrataenium

Pi 0.0239

MarkerSeek score 65.00

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: Genome
length_bp: 220
variable_sites: 5
pi: 0.0239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AATGAACTCCGGCCTTCATC	GCCTTTCAAACAATTTCCACG	50.0	42.9	58.0	56.9	337-346 bp	1.0	66.4
rps2-rpoC2_p2	CCGGCCTTCATCATCTCTTC	GCCTTTCAAACAATTTCCACG	55.0	42.9	58.1	56.9	329-338 bp	1.0	65.8
rps2-rpoC2_p3	CGGCCTTCATCATCTCTTCC	GCCTTTCAAACAATTTCCACG	55.0	42.9	58.1	56.9	328-337 bp	1.0	65.8
rps2-rpoC2_p4	AATGAACTCCGGCCTTCATC	GCCTTTCAAACAATTTCCACGA	50.0	40.9	58.0	58.3	337-346 bp	1.0	65.1
rps2-rpoC2_p5	CGGCCTTCATCATCTCTTCC	GCCTTTCAAACAATTTCCACGA	55.0	40.9	58.1	58.3	328-337 bp	1.0	64.6