Hotspot

atpF-atpH

Genome · 12118-12456 · 339 bp

Back to Tetrataenium

Pi 0.0162

MarkerSeek score 64.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: Genome
length_bp: 339
variable_sites: 5
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	429-455 bp	1.0	82.0
atpF-atpH_p2	CCAGTGGCCTAAAGAAACGA	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.8	58.2	402-428 bp	1.0	81.5
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	431-457 bp	1.0	80.6
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TGAGGCGGAGGGAAAAATAC	50.0	50.0	57.9	57.6	500-526 bp	1.0	80.5
atpF-atpH_p5	CCAGTGGCCTAAAGAAACGA	TGAGGCGGAGGGAAAAATAC	50.0	50.0	57.8	57.6	473-499 bp	1.0	80.1