Hotspot

rpl32-trnL-UAG

SSC · 118232-119230 · 999 bp

Back to Tetrastigma

Pi 0.0215

MarkerSeek score 76.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 999
variable_sites: 109
pi: 0.0215
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTGCGCCAACTAAATAATCA	TTTAGAAGATGCCGCCACTC	38.1	50.0	56.2	58.0	1120-1366 bp	1.0	68.2
rpl32-trnL-UAG_p2	TGTGCGCCAACTAAATAATCA	TCCTAAGAGCAGCGTGTCTA	38.1	50.0	56.2	57.9	1070-1316 bp	1.0	67.8
rpl32-trnL-UAG_p3	TGTGCGCCAACTAAATAATCA	AGCAGCGTGTCTACCAATTT	38.1	45.0	56.2	57.8	1063-1309 bp	1.0	67.4
rpl32-trnL-UAG_p4	TGTGCGCCAACTAAATAATCA	CCTAAGAGCAGCGTGTCTAC	38.1	55.0	56.2	57.8	1069-1315 bp	1.0	67.3
rpl32-trnL-UAG_p5	TGTGCGCCAACTAAATAATCA	CTAAGAGCAGCGTGTCTACC	38.1	55.0	56.2	57.8	1068-1314 bp	1.0	67.3