Hotspot

psbZ-trnG-GCC

LSC · 39457-40123 · 667 bp

Back to Tetrastigma

Pi 0.0278

MarkerSeek score 76.90

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 667
variable_sites: 78
pi: 0.0278
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CCGGTACATCATTATGGATTGGA	AATCGAACCCGCATCTTCTC	43.5	50.0	58.1	58.1	527-818 bp	1.0	66.8
psbZ-trnG-GCC_p2	CCGGTACATCATTATGGATTGGA	GAATCGAACCCGCATCTTCT	43.5	50.0	58.1	58.1	528-819 bp	1.0	66.8
psbZ-trnG-GCC_p3	TCCGGTACATCATTATGGATTGG	AATCGAACCCGCATCTTCTC	43.5	50.0	58.1	58.1	528-819 bp	1.0	66.8
psbZ-trnG-GCC_p4	TCCGGTACATCATTATGGATTGG	GAATCGAACCCGCATCTTCT	43.5	50.0	58.1	58.1	529-820 bp	1.0	66.8
psbZ-trnG-GCC_p5	CCGGTACATCATTATGGATTGG	AATCGAACCCGCATCTTCTC	45.5	50.0	56.7	58.1	527-818 bp	1.0	65.2