Hotspot

trnS-UGA-psbZ

LSC · 38904-39267 · 364 bp

Back to Tetrastigma

Pi 0.015

MarkerSeek score 60.60

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 364
variable_sites: 28
pi: 0.015
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.1	57.0	426-446 bp	1.0	80.6
trnS-UGA-psbZ_p2	CTATCAACCACTCGGCCATC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.1	57.0	415-435 bp	1.0	80.3
trnS-UGA-psbZ_p3	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGAA	55.0	38.1	58.1	57.7	426-446 bp	1.0	78.4
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	GGAGAAGCAAATACAACGGG	55.0	50.0	58.1	56.8	482-502 bp	0.941176	75.2
trnS-UGA-psbZ_p5	CTATCAACCACTCGGCCATC	GGAGAAGCAAATACAACGGG	55.0	50.0	58.1	56.8	471-491 bp	0.941176	74.8