Hotspot

trnE-UUC-trnT-GGU

LSC · 33925-34830 · 906 bp

Back to Tetrastigma

Pi 0.0164

MarkerSeek score 69.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 77
pi: 0.0164
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TCCTTTCTTCCTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	1027-1079 bp	1.0	89.6
trnE-UUC-trnT-GGU_p2	TCCTTTCTTCCTACCCCCAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.0	58.1	1006-1058 bp	1.0	89.2
trnE-UUC-trnT-GGU_p3	TCCTTTCTTCCTACCCCCAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.0	58.1	1003-1055 bp	1.0	89.1
trnE-UUC-trnT-GGU_p4	TCCTTTCTTCCTACCCCCAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.0	58.2	1024-1076 bp	1.0	88.4
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	991-1043 bp	1.0	87.9