Hotspot

psbM-trnD-GUC

LSC · 32010-33180 · 1171 bp

Back to Tetrastigma

Pi 0.021

MarkerSeek score 74.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1171
variable_sites: 132
pi: 0.021
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGCGAGAATATTTACTTCCA	TACGCCTTATTTCCCTGGGA	35.0	50.0	52.0	58.1	1257-1298 bp	1.0	52.0
psbM-trnD-GUC_p2	TGCGAGAATATTTACTTCCA	CGCCTTATTTCCCTGGGATT	35.0	50.0	52.0	57.6	1255-1296 bp	1.0	52.0
psbM-trnD-GUC_p3	ATGCGAGAATATTTACTTCCA	TACGCCTTATTTCCCTGGGA	33.3	50.0	52.7	58.1	1258-1299 bp	1.0	52.0
psbM-trnD-GUC_p4	TGCGAGAATATTTACTTCCAT	TACGCCTTATTTCCCTGGGA	33.3	50.0	52.7	58.1	1257-1298 bp	1.0	52.0
psbM-trnD-GUC_p5	TGCGAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	35.0	50.0	52.0	58.5	1231-1272 bp	1.0	52.0