Hotspot

trnS-GCU-trnR-UCU

LSC · 9448-11450 · 2003 bp

Back to Tetrastigma

Pi 0.0148

MarkerSeek score 65.30

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 2003
variable_sites: 165
pi: 0.0148
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ATTAGCAATCCGCCGCTTTA	TGTCCTATCCATTAGACAATG	45.0	38.1	58.0	52.4	1995-2094 bp	1.0	50.5
trnS-GCU-trnR-UCU_p2	GCTTTAGTCCACTCAGCCAT	TGTCCTATCCATTAGACAATG	50.0	38.1	57.6	52.4	1981-2080 bp	1.0	50.5
trnS-GCU-trnR-UCU_p3	AGTCCACTCAGCCATCTCTC	TGTCCTATCCATTAGACAATG	55.0	38.1	58.5	52.4	1976-2075 bp	1.0	50.5
trnS-GCU-trnR-UCU_p4	ACGGAAAGAGAGGGATTCGA	TGTCCTATCCATTAGACAATG	50.0	38.1	58.2	52.4	2044-2143 bp	1.0	50.4
trnS-GCU-trnR-UCU_p5	AACGGAAAGAGAGGGATTCG	TGTCCTATCCATTAGACAATG	50.0	38.1	57.3	52.4	2045-2144 bp	1.0	50.4