Hotspot

trnH-GUG-psbA

LSC · 90-402 · 313 bp

Back to Tetrastigma

Pi 0.0269
MarkerSeek score 68.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
313
variable_sites
42
pi
0.0269
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCGGCGGTTCACTATTCAG 55.0 50.0 58.1 58.0 768-799 bp 1.0 88.3
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCGGCGGTTCACTATTCAG 50.0 50.0 57.9 58.0 782-813 bp 1.0 88.2
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCGGCGGTTCACTATTCAG 50.0 50.0 57.9 58.0 781-812 bp 1.0 88.2
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCGGCGGTTCACTATTCAG 50.0 50.0 57.9 58.0 783-814 bp 1.0 88.2
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TCAGTGCTATGCATGGTTCC 55.0 50.0 58.1 58.0 752-783 bp 1.0 88.1