Hotspot

ycf3

LSC · 52924-53427 · 504 bp

Back to Tetraselmis

Pi 0.409

MarkerSeek score 80.70

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 504
variable_sites: 198
pi: 0.409
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	GTACTCTTGTGGGTACTGCG	TTGAACCAACGCGTCCTAAA	55.0	45.0	58.0	58.0	1078 bp	0.5	59.5
ycf3_p2	GTACTCTTGTGGGTACTGCG	TTTGAACCAACGCGTCCTAA	55.0	45.0	58.0	58.0	1079 bp	0.5	59.5
ycf3_p3	GTACTCTTGTGGGTACTGCG	CTCTGCCATAGGTGCCATTT	55.0	50.0	58.0	57.9	1024 bp	0.5	59.4
ycf3_p4	GTACTCTTGTGGGTACTGCG	AGTACACTGCAATCGACCAC	55.0	50.0	58.0	57.9	1043 bp	0.5	59.3
ycf3_p5	GTACTCTTGTGGGTACTGCG	GTACACTGCAATCGACCACT	55.0	50.0	58.0	57.9	1042 bp	0.5	59.3