Hotspot

ycf1

IRb · 66369-68744 · 2376 bp

Back to Tetraselmis

Pi 0.362

MarkerSeek score 80.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 2376
variable_sites: 833
pi: 0.362
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAAGATGACACTCGGGCAAG	GCCTGGGGTTCATATTGGAG	50.0	55.0	57.3	58.0	2848 bp	0.5	54.5
ycf1_p2	ACGTGCTCTAGTCCACTGAG	GCCTGGGGTTCATATTGGAG	55.0	55.0	58.8	58.0	2792 bp	0.5	53.3
ycf1_p3	TAAGATGACACTCGGGCAAG	TAGAAAAAGCGGATCGGGTC	50.0	50.0	57.3	57.7	2553 bp	0.5	52.2
ycf1_p4	TTAAGATGACACTCGGGCAAG	GCCTGGGGTTCATATTGGAG	47.6	55.0	58.0	58.0	2849 bp	0.5	51.7
ycf1_p5	TAAGATGACACTCGGGCAAG	AGCCTGGGGTTCATATTGGA	50.0	50.0	57.3	58.4	2849 bp	0.5	51.5