Hotspot

psbD

LSC · 60051-61109 · 1059 bp

Back to Tetraselmis

Pi 0.414

MarkerSeek score 81.10

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1059
variable_sites: 428
pi: 0.414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	TGTGGGTGGATTTGGTCAAA	AGCACCAGCCCAAAATACAA	45.0	45.0	57.5	58.0	1923 bp	0.5	56.2
psbD_p2	TGTGGGTGGATTTGGTCAAA	CCACCAAGCAAAACCTGTTG	45.0	50.0	57.5	58.1	1833 bp	0.5	55.8
psbD_p3	TGTGGGTGGATTTGGTCAAA	CGCCGACAGTTAAAGTACCA	45.0	50.0	57.5	57.9	1798 bp	0.5	55.8
psbD_p4	TGTGGGTGGATTTGGTCAAA	AAACGAACATCTCCACCACC	45.0	50.0	57.5	58.1	2318 bp	0.5	55.4
psbD_p5	TGTGGGTGGATTTGGTCAAA	TCCTGGACTTACTGTTGGGT	45.0	50.0	57.5	57.9	2346 bp	0.5	55.4