Hotspot

psaB

LSC · 2515-4719 · 2205 bp

Back to Tetraselmis

Pi 0.449

MarkerSeek score 70.20

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 416
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.42
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	GGACAACCTGGGCATTCTTT	GCAGGACTTTCCATAAGCGA	50.0	50.0	58.4	58.0	2930 bp	0.5	56.9
psaB_p2	GGACAACCTGGGCATTCTTT	AGCAGGACTTTCCATAAGCG	50.0	50.0	58.4	58.0	2931 bp	0.5	56.8
psaB_p3	CGGTGTTGCCCACTACTTAT	GCAGGACTTTCCATAAGCGA	50.0	50.0	57.6	58.0	2962 bp	0.5	56.6
psaB_p4	CGGTGTTGCCCACTACTTAT	AGCAGGACTTTCCATAAGCG	50.0	50.0	57.6	58.0	2963 bp	0.5	56.6
psaB_p5	TTGTCAAGTATCAGCGTGGG	AGTTACCACTTAGGGCTGGA	50.0	50.0	57.9	57.7	2913 bp	0.5	56.6