Hotspot

petA

LSC · 58535-59494 · 960 bp

Back to Tetraselmis

Pi 0.429

MarkerSeek score 81.30

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 960
variable_sites: 401
pi: 0.429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	ACTGATGCGGGAAACACATT	TCGAATAAACCGCGCTTTTC	45.0	45.0	58.1	57.5	2220 bp	0.5	55.2
petA_p2	ACTGATGCGGGAAACACATT	CGAATAAACCGCGCTTTTCT	45.0	45.0	58.1	57.5	2219 bp	0.5	55.1
petA_p3	CGAAAGTTACTGGCACTCCA	TCGAATAAACCGCGCTTTTC	50.0	45.0	57.8	57.5	1882 bp	0.5	54.6
petA_p4	CGAAAGTTACTGGCACTCCA	CGAATAAACCGCGCTTTTCT	50.0	45.0	57.8	57.5	1881 bp	0.5	54.5
petA_p5	AGCGAAAGTTACTGGCACTC	TCGAATAAACCGCGCTTTTC	50.0	45.0	58.2	57.5	1884 bp	0.5	54.3