Hotspot

rbcL-trnH-GTG

LSC · 51008-51434 · 427 bp

Back to Tetraselmis

Pi 0.384
MarkerSeek score 79.70
Primer available yes
Label rbcL-trnH-GTG

Feature Metadata

label_name
rbcL-trnH-GTG
feature_type
igs
region
LSC
length_bp
427
variable_sites
154
pi
0.384
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-trnH-GTG_p1 TGCAATGCACGCAGTAATTG CGTTAGGTCTTGGACTTCCG 45.0 55.0 58.0 58.0 1544 bp 0.5 59.8
rbcL-trnH-GTG_p2 GCGTGTACTCAAGCTCGTAA CGTTAGGTCTTGGACTTCCG 50.0 55.0 58.0 58.0 1153 bp 0.5 59.7
rbcL-trnH-GTG_p3 AGCAGCTTGTGAAGTTTGGA CGTTAGGTCTTGGACTTCCG 45.0 55.0 57.9 58.0 1061 bp 0.5 59.5
rbcL-trnH-GTG_p4 TCGTAACCTTGCTCGTGAAG CGTTAGGTCTTGGACTTCCG 50.0 55.0 57.9 58.0 1127 bp 0.5 59.5
rbcL-trnH-GTG_p5 TGTTATTCGTGCGGCTTGTA CGTTAGGTCTTGGACTTCCG 45.0 55.0 57.9 58.0 1100 bp 0.5 59.3